| Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt | | 1 | 209 | Mon Aug 03 2026 |
| Cutadapt trim-paired with separate 5'/3' adapters retaining primers — is switching to linked adapters the correct fix? | | 1 | 40 | Tue Jul 21 2026 |
| Minimum length threshold in cutadapt for marker of variable length | | 9 | 143 | Mon Jul 20 2026 |
| High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles) | | 2 | 112 | Thu Apr 09 2026 |
| Problem with cache using qiime cutadapt | | 8 | 97 | Mon Mar 30 2026 |
| Q2-cutadapt add "--nextseq-trim" option | | 3 | 126 | Fri Feb 27 2026 |
| Possible bug in qiime cutadapt demux paired (2024.10) | | 3 | 98 | Wed Jan 28 2026 |
| Huge difference of DADA2 output with and without removing primers | | 4 | 121 | Tue Jan 27 2026 |
| qiime cutadapt does not have an untrimmed sequences output | | 4 | 144 | Mon Dec 01 2025 |
| ITS denoising truncation | | 1 | 69 | Wed Oct 15 2025 |
| Duplicate barcode error, but barcodes listed are not the same. | | 6 | 106 | Tue Aug 19 2025 |
| cutadapt outside of QIIME2 | | 1 | 70 | Mon Aug 04 2025 |
| Inconsistent results with discard untrimmed reads in cutadapt | | 6 | 166 | Thu Jul 10 2025 |
| QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis | | 13 | 535 | Thu Apr 17 2025 |
| Analysing V4/V5 16S rRNA illumina Miseq paired-end reads. | | 15 | 461 | Tue Mar 18 2025 |
| Trimming Paired-End Sequences with cutadapt While Maintaining Pairing Integrity | | 1 | 95 | Mon Feb 10 2025 |
| Which position is best for trimming in a paired-end approach? | | 10 | 174 | Fri Jan 24 2025 |
| Comparing amplicon studies that use different primers | | 6 | 204 | Wed Nov 20 2024 |
| Trimming 5bp in QIIME2 2024.5 Amplicon | | 3 | 56 | Thu Nov 14 2024 |
| Error messages after cutadapt, but got trimmed files. | | 4 | 220 | Thu Nov 07 2024 |
| Cut adapt results in empty files | | 3 | 144 | Fri Oct 18 2024 |
| One vs two adaptor sequences using cutadapt | | 4 | 114 | Fri Sep 27 2024 |
| QIIME2 Demultiplexing Sequence | | 6 | 230 | Tue Sep 03 2024 |
| V3-V4 16s - variable size after merging | | 6 | 280 | Mon Aug 05 2024 |
| problem with cutadapt trim-single | | 3 | 150 | Tue Jul 02 2024 |
| Removal of Illumina Adapters | | 1 | 208 | Thu May 30 2024 |
| Error during deblur process | | 7 | 328 | Sat May 11 2024 |
| Cutadapt - trimming adaptor and primer sequences | | 2 | 241 | Sat May 11 2024 |
| Missing plugins to load Artifact to run cut adapt | | 1 | 150 | Tue May 07 2024 |
| Primer show up again after removal using cutadapt in qiime2 DADA2 | | 9 | 429 | Mon May 06 2024 |
